Use of NESTROFT as a screening test for the carriers of thalassemia major
Abstract
Objective: To evaluate use of NESTROFT as a screening test for the carrier of thalassemia major.
Design: prospective study. Settings and participants: 121 random clinically normal children who were siblings of thalassemia major patients who registered at thalassemia welfare society of J K LON hospital kota. Intervention: All cases were investigated for CBC, NESTROFT and HbA2 estimation by HPLC method.
Result: Total 121 cases were screened for carrier by HPLC method for HbA2 estimation. Total 59 (48.76%) cases had HbA2 level ≥3.5 %, considered as carrier while 62 (51.24%) cases were noncarrier. The Sensitivity, specificity, PPV and NPV of MCV<80, Mentzer index (<13 were positive), and NESTORFT, were 89.83%, 46.77,61.62% ,82.85% ; 83.05%, 97.36%, 96.07% ,88.09 % and 93.22%, 88.70, 88.7 and 93.22 % respectively. So Nestorft is better test (sensitivity-93.22% and NPV is 93.22%) for screening for carrier.
Conclusion: It is concluded that β thalassemia carriers are more prevalent in siblings of thalassemia major. For screening NESTORF test is reliable and cost effective and can be used for mass hemoglobinopathies screening programmes.
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