A cross sectional study of in-hospital cases of Pediatric Tuberculosis detected by CBNAAT at a tertiary care teaching hospital of Central India.

  • Dr. Munindra Pratap Singh Demonstrator, Department of Physiology, G.R. Medical College Gwalior (M.P.), India
  • Dr. Pawan Kumar Sharma Associate Professor (Designated), Govt. Medical College, Datia (M.P.), India
  • Dr. Pradeep Upadhyay Child Specialist, Govt. Medical College Associated District Hospital Datia (M.P.), India
  • Dr. Puneet Agrawal Senior Resident, Department of Pediatrics, Govt. Medical College, Datia (M.P.), India
  • Dr. Dinesh Kumar Gupta Child Specialist, Govt. Medical College associated District Hospital Datia (M.P.), India
  • Dr. Vishal Verma District Tuberculosis Officer, Datia (M.P.), India
  • Dr. Abhishek Mehta Associate Professor, Department of Microbiology, Govt. Medical College, Datia (M.P.), India
  • Dr. Rajesh Gupta Professor, Department of Pediatrics, Govt. Medical College, Datia (M.P.), India
Keywords: Pediatric TB, CBNAAT, Gastric aspirate, Sputum


Background: Childhood tuberculosis is difficult in the identification of the organism due to improper sampling as well as low sensitivity of the smear. Newer diagnostic methods like Cartridge based nucleic acid amplification tests (CBNAAT) can rapidly identify Mycobacterium tuberculosis with improved sensitivity over the smear testing.

Material & Method: This observational record based cross-sectional study was undertaken to identify the epidemiology of tubercular infection in children diagnosed with CBNAAT. The study was carried out by analyzing the data of children from six months to 18 years who were diagnosed with Tuberculosis and treated with anti-tuberculosis therapy (ATT) from April 2018 to March 2020.

Results: Data of a total of 166 patients was analyzed. 42% of overall collected samples were reactive to CBNAAT testing for tuberculosis. 40 gastric aspirate samples were collected and only four (10%) turned reactive for tuberculosis by CBNAAT. None of the pediatric samples was positive for MDR TB. 66% of children completed treatment and 33% were declared cured.

Conclusion: 42% positivity after CBNAAT testing for tuberculosis infection in collected samples of sputum and gastric aspirate where only 10% yield in GA samples.


Download data is not yet available.


WHO Global Tuberculosis Report 2020.https://www.who.int/teams/global-tuberculosis-programme/data accessed on 07-12-2020

Singh V. Pediatric TB Management Under RNTCP: What and Why? Indian J Pediatr. 2019 Aug;86(8):707-713. DOI: 10.1007/s12098-019-03001-7

Carvalhoa I, Goletti D, Mangac S, Silva DR, et al. Managing latent tuberculosis infection and tuberculosis in children. Pulmonol. 2018; 24(2):106---114. DOI: 10.1016/j.rppnen.2017.10.007

Sahana KS, Prabhu AS, Saldanha PRM. Usage of Cartridge Based Nucleic Acid Amplifification Test (CB-NAAT/GeneXpert) test as diagnostic modality for pediatric tuberculosis; case series from Mangalore, South India. J Clin Tuberc Other Mycobact Dis. 2018; 11:7–9. DOI: 10.1016/j.jctube.2017.12.002

Tuberculosis in Childhood. Delane Shingadia.. Ther Adv Respir Dis 2012: 6(3) 161 –171. https://doi.org/10.1177%2F1753465812436662

https://tbfacts.org/tb-statistics-india/ accessed on 17-06-2020

https://www.who.int/publications/guidelines/en/ accessed on 16-08-2020

https://reports.nikshay.in/Reports/TBNotification accessed on 16-08-2020

Belgaumkar V, Chandanwale A, Valvi C et al. Barriers to screening and isoniazid preventive therapy for child contacts of tuberculosis patients. Int J Tuberc Lung Dis. 2018. 1;22(10):1179-1187. DOI: 10.5588/ijtld.17.0848

Jain SK, Ordonez A, Kinikar A et.al. Pediatric Tuberculosis in Young Children in India: A Prospective Study. BioMed Research International. 2013. https://doi.org/10.1155/2013/783698

Raviglione M., Snider D. , Kochi A. Global epidemiology of tuberculosis JAMA.1995; 273: 220–226. doi:10.1001/jama.1995.03520270054031

Sharma SK, Kohli M, Yadav RN, et al. Evaluating the diagnostic accuracy of xpert MTB/RIF assay in pulmonary tuberculosis. PLoS ONE 2015;10(10). https://doi.org/10.1371/journal.pone.0141011.

Swingler GH, du Toit G, Andronikou S, van der ML, Zar HJ. Diagnostic accuracy of chest radiography in detecting mediastinal lymphadenopathy in suspected pulmonary tuberculosis. Arch Dis Child 2005;90:1153–1156. doi: 10.1136/adc.2004.062315

Zar HJ, Hanslo D, Apolles P, Swingler G, Hussey G. Induced sputum versus gastric lavage for microbiological confirmation of pulmonary tuberculosis in infants and young children: a prospective study. Lancet 2005;365:130–134. doi: 10.1016/S0140-6736(05)17702-2.

Detjen AK, DiNardo AR, Leyden J, Steingart KR, Menzies D, Schiller I, et al. Xpert MTB/RIF assay for the diagnosis of pulmonary tuberculosis in children: a systematic review and meta-analysis. Lancet Respir Med. (2015) 3:451–61. doi: 10.1016/S2213-2600(15)00095-8.

Maciel EL, Brotto LD, Sales CM et al. Gastric lavage in the diagnosis of pulmonary tuberculosis in children: a systematic review. Rev Saúde Pública 2010;44(4). DOI: 10.1590/s0034-89102010005000019

Iram S, Zeenat A, Hussain S, Wasim Yusuf N, Aslam M. Rapid diagnosis of tuberculosis using Xpert MTB/RIF assay Report from a developing country. Pak J Med Sci 2014;31:105 10. DOI: 10.12669/pjms.311.6970

Sharma S, Shulania A, Achra A, et al. Diagnosis of pulmonary tuberculosis from gastric aspirate samples in nonexpectorating pediatric patients in a tertiary care hospital. Indian J Pathol Microbiol 2020;63:210-3.

DOI: 10.17511/ijpr.2021.i01.06
Published: 2021-02-28
How to Cite
Dr. Munindra Pratap Singh, Dr. Pawan Kumar Sharma, Dr. Pradeep Upadhyay, Dr. Puneet Agrawal, Dr. Dinesh Kumar Gupta, Dr. Vishal Verma, Dr. Abhishek Mehta, & Dr. Rajesh Gupta. (2021). A cross sectional study of in-hospital cases of Pediatric Tuberculosis detected by CBNAAT at a tertiary care teaching hospital of Central India. Pediatric Review: International Journal of Pediatric Research, 8(1), 39-44. https://doi.org/10.17511/ijpr.2021.i01.06
Original Article