Diagnosis of dengue- an overview

  • Dr. Rabindran Billroth Hospital, Chennai, India
  • Dr. D. Sharad Gedam R. K. D. F. Medical College Associated S. R. K. University, Bhopal, MP, India.
Keywords: Dengue, MAC-ELISA, Biosensors

Abstract

The first investigation ordered on clinical suspicion of dengue is complete blood count. The classical dengue triad includes increased hematocrit, atypical plasmacytoidlymphocytosis and thrombocytopenia [1].Hemoconcentration (hematocrit elevated > 45% or by > 20% from previous value) is diagnostic of Dengue Hemorrhagic Fever. Further confirmation is usually made with Dengue Antigen test. During early stages of the disease around 4-5 days of fever onset, virus isolation, nucleic acid or antigen detection are used to diagnose the virus from serum, plasma or tissues. However serology remains the method of choice during convalescence. Other tests include antibody titre for dengue virus types, Polymerase chain reaction (PCR), Liver function tests, Serum protein & albumin levels & Coagulation panel.

Downloads

Download data is not yet available.

References

1. Wiwanitkit V. Dengue fever: diagnosis and treatment. Expert Rev Anti Infect Ther. 2010 Jul;8(7):841-5. doi: 10.1586/eri.10.53. [PubMed]

2. Kowitdamrong E, Thammaborvorn R, Semboonlor L, Mungmee V, Bhattarakosol P. Detection of dengue HI and IgM antibody: is it diagnostically useful? when and how? J Med Assoc Thai. 2001 Jun;84 Suppl1:S148-54. [PubMed]

3. VirojWiwanitkit. The Importance of Accurate Diagnosis of Dengue Fever; Future Virology. 2012;7(1):53-62.

4. Peh AE, Leo YS, Toh CS. Current and nano-diagnostic tools for dengue infection. Front Biosci (Schol Ed). 2011 Jun 1;3:806-21.

5. Dinesh Subedi, Andrew W. Taylor-Robinson. Laboratory Diagnosis of Dengue Infection: Current Techniques and Future Strategies. Open Journal of Clinical Diagnostics; Vol.4 No.1(2014), Article ID:44045,8 pages. DOI:10.4236/ojcd.2014.41012.

6. Shu PY, Huang JH. Current advances in dengue diagnosis. ClinDiagn Lab Immunol. 2004 Jul;11(4):642-50. [PubMed]

7. Henchal EA, Gentry MK, McCown JM, Brandt W E. Dengue virus-specific and flavivirus group determinants identified with monoclonal antibodies by indirect immunofluorescence. Am J Trop Med Hyg. 1982 Jul;31(4):830-6. [PubMed]

8. Simmons CP, Farrar JJ, Nguyen vV, Wills B. Dengue. N Engl J Med. 2012 Apr 12;366(15):1423-32. doi: 10.1056/NEJMra1110265. [PubMed]

9. Ranjit S, Kissoon N. Dengue hemorrhagic fever and shock syndromes. PediatrCrit Care Med. 2011 Jan;12(1):90-100. doi: 10.1097/PCC.0b013e3181e911a7. [PubMed]

10. Zhang Yi, Bai J, Ying J. A stacking flow immunoassay for the detection of dengue-specific immunoglobulins in salivary fluid. Royal Society of Chemistry. Lab on a Chip. 2015(6);doi: 10.1039/c4lc01127a.

11. C. A. M. van de Weg, R. M. H. G. Huits, C. S. Pannuti et al., Hyperferritinaemia in Dengue Virus Infected Patients Is Associated with Immune Activation and Coagulation Disturbances. PLoS Neglected Tropical Diseases, vol. 8, no. 10, 2014. doi.org/10.1371/journal.pntd.0003214.

12. Khandelwal R, Khandelwal LM. Effect of dengue fever on the total leucocyte count and neutrophil count in children in early febrile period. Int J Pediatr Res. 2017;4(10):617-622.doi:10. 17511/ijpr.2017.10.06.
Diagnosis of dengue- an overview
CITATION
DOI: 10.17511/ijpr.2017.i10.01
Published: 2017-10-31
How to Cite
Dr. Rabindran, & Dr. D. Sharad Gedam. (2017). Diagnosis of dengue- an overview. Pediatric Review: International Journal of Pediatric Research, 4(10), 588-589. https://doi.org/10.17511/ijpr.2017.i10.01
Section
Editorial